Protocol for using recombinant Cas9 Nuclease to assess locus modification in genome editing experiments | NEB
In vitro Generation of CRISPR-Cas9 Complexes with Covalently Bound Repair Templates for Genome Editing in Mammalian Cells
Preparation of Cas9 Ribonucleoproteins for Genome Editing
CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish - ScienceDirect
Co-expression of Cas9 and single-guided RNAs in Escherichia coli streamlines production of Cas9 ribonucleoproteins | Communications Biology
Preparation of Cas9 Ribonucleoproteins for Genome Editing
Directed evolution of CRISPR-Cas9 to increase its specificity | Nature Communications
CRISPR-Cas9-induced gene knockout in zebrafish
Frontiers | A CRISPR/Cas9-Based Mutagenesis Protocol for Brachypodium distachyon and Its Allopolyploid Relative, Brachypodium hybridum
A simple protocol to isolate a single human cell PRDX1 knockout generated by CRISPR-Cas9 system - ScienceDirect
MPs | Free Full-Text | A Two-Step Method for Obtaining Highly Pure Cas9 Nuclease for Genome Editing, Biophysical, and Structural Studies
CRISPR-Cas9 Protein – TriAltus Bioscience
An efficient and cost-effective purification protocol for Staphylococcus aureus Cas9 nuclease - ScienceDirect
CRISPR-Cas9-induced gene knockout in zebrafish - ScienceDirect
MPs | Free Full-Text | A Robust Expression and Purification Method for Production of SpCas9-GFP-MBP Fusion Protein for In Vitro Applications
Engineering a far-red light–activated split-Cas9 system for remote-controlled genome editing of internal organs and tumors | Science Advances
Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 in vitro transcripts or ribonucleoproteins | Nature Protocols
Frontiers | Rapid Assessment of CRISPR Transfection Efficiency and Enrichment of CRISPR Induced Mutations Using a Dual-Fluorescent Stable Reporter System
Optimized protocol to create deletion in adherent cell lines using CRISPR/ Cas9 system - ScienceDirect
CRISPR-Cas9/Cas12A expression, purification and validation - Genome Innovation Hub - University of Queensland
Introduction to the CRISPR/Cas9 system
An efficient and cost-effective purification protocol for Staphylococcus aureus Cas9 nuclease - ScienceDirect
CRISPR–Cas9-mediated genome editing in apple and grapevine | Nature Protocols
MPs | Free Full-Text | A Two-Step Method for Obtaining Highly Pure Cas9 Nuclease for Genome Editing, Biophysical, and Structural Studies
Purification of Cas9 protein. (A) SDS-PAGE (sodium dodecyl sulfate... | Download Scientific Diagram
A Expression of the recombinant Cas9-GFP protein, B purification of... | Download Scientific Diagram
A detailed protocol for expression, purification, and activity determination of recombinant SaCas9 - ScienceDirect
